# Hi, I'm David 🥽💊  **From left: my husband, Paul; our mini aussie, Dodger; and I.** <br> ​I have served as an early-career medical professional with **over 5 years of experience** across both laboratory medicine and pharmacy. I am actively advancing my role as a **Medical Laboratory Technician** (ASCP) and **Certified Pharmacy Technician** (CPhT) to further distinguish myself in these high-impact fields. I enjoy technical writing, reading journal papers, creating eye-candy science art, and working in the clinical lab. > #### My mission is to deploy Cryo-Electron Microscopy (CryoEM) to examine the shape, purpose, and how a cell's tools, called ==proteins==, work. Leveraging this data, while sometimes existentially exciting, continues to help countless lives. This is the basic science of **Structural Biology** put short and it [has a rich history](https://youtu.be/bHyi7bIzlfo?si=lCEyFv8nG7XgsJX7). To locate future roles in this field I'm finding time to shadow a transmission electron microscope (TEM) in central Ohio for sufficient scoping practice to one day specialize in CryoEM. This microscopy technique reconstructs the contents of cells and digitizes them on a computer. It's a labor-heavy process, but you can see it explained in under a minute below:  # Projects ## Science Illustration I make sense of researcher data by visualizing key parts of biomolecules that plays nicely with our eyes. <iframe src="https://3dproteinimaging.com/protein-imager/?projectId=cpwvYg6VmtptHkCoLWSm&isUserProject=1&gui=transparent" width="100%" height="400"></iframe> [CaMKII](https://www.rcsb.org/structure/3SOA) is a star-shape protein that tunes the heart's pacemaking in our fight-or-flight response^[Schulman H, Anderson ME. Ca2+/calmodulin-dependent protein kinase II in heart failure. Drug Discovery Today: Disease Mechanisms. 2010 Jun 1;7(2):e117-22. https://doi.org/10.1016/j.ddmec.2010.07.005] and it's tied to learning and memory in our brains.^[Zalcman G, Federman N, Romano A. CaMKII isoforms in learning and memory: localization and function. Frontiers in molecular neuroscience. 2018 Dec 4;11:445. https://doi.org/10.3389/fnmol.2018.00445] Web-app, [Protein Imager](https://3dproteinimaging.com/), developed by Tomasello, Armenia, and Molla.^[Tomasello G, Armenia I, Molla G. The Protein Imager: a full-featured online molecular viewer interface with server-side HQ-rendering capabilities. Bioinformatics. 2020 May 1;36(9):2909-11. https://doi.org/10.1093/bioinformatics/btaa009] Special thanks to Tomasello for [iframe](https://developer.mozilla.org/en-US/docs/Web/HTML/Reference/Elements/iframe) support. ## Technical Writing ### Research Review I'm filling in my knowledge gaps by writing a manuscript over [Basic Protein Science](Basic%20Protein%20Science.md). This self-study serves as a review for my future studies as a Technologist in Molecular Biology, or MB(ASCP). ### CPhT Prep I'm happy to share [what to study to obtain an Ohio CPhT license](PTCE.md) in 2025 created with good friends that are PTCB registered and certified (RPhT/CPhT). # Contact **@lemasterdj** [ORCID](https://orcid.org/0009-0000-3548-3303) [GitHub](https://github.com/lemasterdj) [Zotero](https://www.zotero.org/lemasterdj) [LinkedIn](https://linkedin.com/in/lemasterdj) [Instagram](https://instagram.com/lemasterdj) **[email protected] (614) 604-2565**